A chromosome-level genome assembly of the striped catfish (Pangasianodon hypophthalmus)

A high-quality genome assembly of a mature female individual of striped catfish was generated by integration of short reads from an Illumina Hiseq Xten platform, long reads from a Nanopore platform, and Hi-C sequencing data. The Illumina short reads were sequenced at 60 X coverage, while the Nanopore long reads covered 85 X of the genome. This assembled genome is 742.6 Mb in length, with a contig N50 of 3.5 Mb and a scaffold N50 of 29.5 Mb, of which a total of 731.7 Mb reads (98.5%) were anchored on 30 pseudo-chromosomes. Among the totally searched 4,584 BUSCO groups in the popular actinopterygii_odb9 database, 4,279 (93.3%) BUSCO core genes were completely identified.

本研究通过整合Illumina Hiseq Xten平台产出的短读长测序reads、Nanopore平台产出的长读长测序reads以及Hi-C测序数据，完成了一条成熟雌性条纹鲶鱼个体的高质量基因组组装。其中，Illumina短读长测序reads的测序深度达60倍，Nanopore长读长测序reads覆盖基因组85倍。该组装基因组总长度为742.6 Mb，重叠群（contig）N50为3.5 Mb，支架（scaffold）N50为29.5 Mb；其中总计731.7 Mb的序列（占比98.5%）被锚定至30条伪染色体上。在常用的硬骨鱼actinopterygii_odb9数据库中检索的4584个BUSCO基因家族中，共完整鉴定出4279个BUSCO核心基因，占比93.3%。