Dataset related to article "The Microbiome of Catheter Collected Urine in Males with Bladder Cancer According to Disease Stage"

This record contains raw data related to article "The Microbiome of Catheter Collected Urine in Males with Bladder Cancer According to Disease Stage" <strong>Purpose: </strong> The dogma that urine is sterile has been overturned and dysbiosis of the urinary microbiome has been linked to many urological disorders. We tested the hypothesis that the urinary microbial composition may be different between men with or without bladder cancer in catheter collected urines, bladder washouts and midstream voided urines, and may be dependent on tumor staging. <strong>Materials and methods: </strong> Liquid samples were collected from male patients with bladder cancer, and sex and age matched nonneoplastic controls. Total DNA was extracted and processed for 16S rRNA gene sequencing. Bioinformatic analysis for microbial classification was performed to assess diversity and variations. <strong>Results: </strong> The urinary microbiome associated with catheter collected urine samples of patients with bladder cancer was characterized by a significantly increased abundance of <em>Veillonella</em> (p=0.04) and <em>Corynebacterium</em> (p=0.03), and decreased <em>Ruminococcus</em> (p=0.03) compared to controls, with differences exacerbating with disease progression. Compared to catheterized urines, bladder cancer washouts showed the specific increase of some taxa, like <em>Burkholderiaceae</em> (p=0.014), whereas midstream urines were enriched in <em>Streptococcus</em> (p &lt;0.0001), <em>Enterococcus</em> (p &lt;0.0001), <em>Corynebacterium</em> (p=0.038) and <em>Fusobacterium</em> (p &lt;0.0001). <strong>Conclusions: </strong> The bladder is colonized by endogenous bacteria and microbial modifications characterize the microbiome of patients with bladder cancer. Different microbial compositions can be characterized by changing sampling strategy. These results pave the way for exploring new diagnostic and therapeutic options based on the manipulation of the bacterial community.本数据集包含与论文《按疾病分期划分的男性膀胱癌患者导尿尿液微生物组》相关的原始数据。 **研究目的**：尿液无菌的固有观念已被推翻，尿液微生物组失调与多种泌尿系统疾病存在关联。我们验证了如下假说：在导尿采集的尿液、膀胱冲洗液以及中段尿样本中，膀胱癌男性患者与非癌对照者的尿液微生物组成存在差异，且该差异可能与肿瘤分期相关。 **材料与方法**：从膀胱癌男性患者以及性别、年龄匹配的非肿瘤对照受试者中采集液体样本。提取总DNA并进行16S rRNA基因测序（16S rRNA gene sequencing）。开展微生物分类的生物信息学分析，以评估微生物群落的多样性与变异情况。 **结果**：与对照组相比，膀胱癌患者导尿样本相关的尿液微生物组具有显著富集的韦荣球菌属（Veillonella）（p=0.04）和棒状杆菌属（Corynebacterium）（p=0.03），以及显著减少的瘤胃球菌属（Ruminococcus）（p=0.03），且上述差异随疾病进展而加剧。与导尿样本相比，膀胱癌患者的膀胱冲洗液样本中存在特定类群的富集，例如伯克霍尔德菌科（Burkholderiaceae）（p=0.014）；而中段尿样本中则富集了链球菌属（Streptococcus）（p<0.0001）、肠球菌属（Enterococcus）（p<0.0001）、棒状杆菌属（Corynebacterium）（p=0.038）以及梭杆菌属（Fusobacterium）（p<0.0001）。 **结论**：膀胱内存在内源细菌定植，膀胱癌患者的微生物组以微生物群落改变为特征。通过调整采样策略，可以区分出不同的微生物组成。本研究结果为探索基于细菌群落调控的新型诊断与治疗方案铺平了道路。