Effect of polyhydroxyalkanoates on the microbial reductive dechlorination of polychlorinated biphenyls and competing anaerobic respiration in a marine microbial culture

The effect of polyhydroxyalkanoates (PHAs) with different composition on the reductive dechlorination activity of a polychlorinated biphenyls (PCBs) dechlorinating marine microbial community and on the activity of sulfate-reducing (SRB) and methanogenic bacteria (MB), were investigated in marine sediment microcosms and compared with the main monomer, 3-hydroxybutyric acid (3HB). Microbial community in marine sediment microcosms was analyzed using 16S rRNA gene Illumina sequence (V3-V4 region) after 89 days of incubation with the different amendments.

本研究以海洋沉积物微宇宙为实验体系，探究不同组成的聚羟基脂肪酸酯（polyhydroxyalkanoates, PHAs）对多氯联苯（polychlorinated biphenyls, PCBs）脱氯海洋微生物群落的还原脱氯活性，以及对硫酸盐还原菌（sulfate-reducing, SRB）和产甲烷菌（methanogenic bacteria, MB）活性的影响，并以其主要单体3-羟基丁酸（3-hydroxybutyric acid, 3HB）作为对照。经不同添加物培养89天后，采用16S rRNA基因Illumina测序（V3-V4区）对海洋沉积物微宇宙中的微生物群落进行分析。