Global identification and systematic analysis of lysine malonylation in maize (Zea mays L.

Lysine malonylation is a kind of post-translational modifications (PTMs) discovered in recent years, which plays an important regulatory role in plants. Maize (Zea mays L.) is a major global cereal crop. We therefore performed a global malonylome analysis of maize. In total, 1722 uniquely malonylated lysine residues were obtained in 810 proteins. The modified proteins were involved in various biological processes such as photosynthesis, ribosome and oxidative phosphorylation. Notably, a large proportion of the modified proteins (45%) were located in chloroplast, suggesting an indispensable regulatory role of malonylation in photosynthesis and carbon fixation.

赖氨酸丙二酰化（Lysine malonylation）是近年来发现的一类蛋白质翻译后修饰（post-translational modifications, PTMs），在植物体内发挥重要调控作用。玉米（Zea mays L.）作为全球主要谷类作物，本研究对其开展了全球丙二酰化修饰组（malonylome）分析。最终在810个蛋白质中鉴定得到1722个独特的丙二酰化赖氨酸残基。这些修饰蛋白广泛参与光合作用、核糖体活动、氧化磷酸化等多种生物学过程。值得关注的是，45%的修饰蛋白定位于叶绿体，提示丙二酰化在光合作用与碳固定过程中具备不可或缺的调控功能。