GBM cell lines derived and propagated according to Cambridge protocol

We screened the gene expression in GBM cell lines cultured under serum-free conditions. We have special interest in genes associated with glial development such as NG2, Olig2 and PDGFRa. This microarray data gave us an idea about the expression of these genes and made the basis for further investigation. Seven GBM cells were derived from 7 different patients under serum-free conditions according to Cambridge protocol as previously described (Fael Al-Mayhani et al, 2009). The RNA was extracted and its quality was assessed. The samples were sent for microarray screening using Illumina platform. The gene was considered as present when the detection value was greater than 0.99.

我们对无血清培养条件下培养的胶质母细胞瘤（Glioblastoma, GBM）细胞系开展了基因表达筛选。本研究重点关注与神经胶质发育相关的基因，如NG2、Olig2及PDGFRα。本次基因芯片（microarray）数据清晰呈现了上述基因的表达情况，为后续研究奠定了坚实基础。按照此前Fael Al-Mayhani等（2009）报道的剑桥实验方案（Cambridge protocol），我们从7名不同患者体内分离得到7株无血清培养的GBM细胞系。随后提取样本总RNA并对其质量进行评估，将样本送至Illumina平台完成基因芯片筛查。当基因的检测值大于0.99时，即判定该基因存在表达。