BXPC3 and PANC1 secretome analysis (SEC61-biotin transferase system)

Cells expressing a ER resident biotin trasferase (SEC61 fusion) secreted biotinylated protein in the culture medium. This project nominated bona fide secreted factors.表达内质网（Endoplasmic Reticulum，ER）驻留生物素转移酶（SEC61融合蛋白）的细胞可在培养基中分泌生物素化蛋白。本项目鉴定出了确凿的分泌因子。