Genomic DNA transposition induced by human PGBD5 (Accompanying Scripts for Computational Analyses)

We include the bash scripts, python code and command-line parameters used to prepare, map, and analyze NGS sequencing data generated from a modified version of flanking sequence exponential anchored (FLEA) PCR to identify genomic insertion locations of a transposable element reporter construct. In summary, we map these reads to a hybrid genome consisting in the human reference hg19 and the reporter plasmid, identify reads that span the insertion breakpoint and thus recover the genomic insertion loci. 

Please find more details in the supplied README. 

This collection of scripts corresponds to the data analysis in the following publication:

Genomic DNA transposition induced by human PGBD5

Anton Henssen, Elizabeth Henaff, Eileen Jiang, Amy Eisenberg, Julianne R. Carson, Camila Villasante, Mondira Ray, Eric Still, Melissa Burns, Jorge Gandara, Cedric Feschotte, Christopher E. Mason, Alex Kentsis

本数据集包含用于制备、比对及分析源自改良版侧翼序列指数锚定（Flanking Sequence Exponential Anchored, FLEA）PCR的下一代测序（Next-Generation Sequencing, NGS）数据的Bash脚本、Python代码与命令行参数，该实验旨在鉴定转座元件报告基因构建体的基因组插入位点。简言之，我们将这些测序读段（reads）比对至由人类参考基因组hg19与报告质粒组成的嵌合基因组，识别跨越插入断点的测序读段，进而获取基因组插入位点。

更多细节请参见提供的README文件。

本脚本集对应于以下发表论文中的数据分析工作：
《人类PGBD5诱导的基因组DNA转座》
Anton Henssen, Elizabeth Henaff, Eileen Jiang, Amy Eisenberg, Julianne R. Carson, Camila Villasante, Mondira Ray, Eric Still, Melissa Burns, Jorge Gandara, Cedric Feschotte, Christopher E. Mason, Alex Kentsis