Fast Repetition Rate Fluorometry (FRRF) data collected on the RV L'Astrolabe from 27/02/2008 to 02/03/2008 (Dumont d'Urville - Hobart)

These data were collected on the RV L'Astrolabe (platform code: FHZI) from 27/02/2008 to 02/03/2008 on a trip from Dumont D'Urville to Hobart.Maximum photochemical efficiency of photosystem II (PSII), also called maximum quantum yield of PSII (Fv/Fm), has become one of the most widely utilized fluorescence parameters in phytoplankton research. It represents the potential photochemical efficiency, which is the probability that the light energy captured by the photosynthetic apparatus is being utilized as photochemistry. Fv/Fm has been shown to have an instant response to variations in physical and chemical properties and is interpreted as a diagnostic of the overall health or competence of phytoplankton. Together with the absorption cross section area of PSII and chlorophyll concentration, it can be used to measure primary production (Cheah et al. 2011, Deep Sea Research).Seawater from 3 m depth was supplied continuously from the ship’s clean seawater line. FRR fluorescence yields were measured continuously at 1 minute intervals in dark-adapted state (! 15 minutes dark-adaptation) using a flash sequence consisting of a series of 100 subsaturation flashlets (1.1 μs flash duration and 2.8 μs interflash period) and a series of 20 relaxation flashlets (1.1 μs flash duration and 51.6 μs interflash period).

本数据集采集于‘阿斯托拉贝号’研究船（RV L'Astrolabe，平台代码：FHZI），采集时段为2008年2月27日至2008年3月2日，采集航次为从迪蒙·迪维尔前往霍巴特的航程。

光系统II（photosystem II, PSII）的最大光化学效率，又称PSII最大量子产额（Fv/Fm），现已成为浮游植物研究中应用最为广泛的荧光参数之一。该参数表征潜在光化学效率，即光合机构捕获的光能用于光化学反应的概率。研究表明，Fv/Fm对水体理化性质变化具有瞬时响应，可作为浮游植物整体健康水平或生理活性的诊断依据。结合光系统II吸收截面与叶绿素浓度，该参数可用于初级生产力的测定（Cheah et al. 2011, Deep Sea Research）。

实验采用船舶洁净海水管路连续抽取3米深度的海水。在暗适应状态（需经15分钟暗适应）下，以1分钟为间隔连续测定快速重复率（Fast Repetition Rate, FRR）荧光产量。测量采用的闪光序列包含两组：100个亚饱和微闪光（闪光时长1.1微秒，闪光间隔2.8微秒），以及20个弛豫微闪光（闪光时长1.1微秒，闪光间隔51.6微秒）。