Additional file 3: of Succession and persistence of microbial communities and antimicrobial resistance genes associated with International Space Station environmental surfaces

Figure S1. Percentage of total reads obtained from PMA and non-PMA treated samples. Figure S2. Proportional abundance at flight level. The proportional abundance of different flight sampling shows the presence of all domains (Bacteria, Archaea, and Eukaryota). There is a noticeable increase in bacterial population from Flight 1 to Flight 3. Figure S3. Top 25 species dominating the microbial composition of ISS samples. Normalized sequence reads were mapped to the reference database at species-level resolution. Image representing the comparison of relative abundance of the species in non-PMA and PMA treated samples during Flight 1 to Flight 3. Figure S4. Abundance of metagenomics reads related to the microbial phylum of PMA treated ISS environmental samples. Figure S5. Abundance of metagenomics reads related to the genus of PMA-treated ISS environmental samples. Figure S6. Abundance of antimicrobial resistance metagenomics reads in PMA- and non-PMA-treated samples from Flight 1, Flight 2, and Flight 3. Figure S7. Abundance of metagenomics reads associated with virulence. (ZIP 180 kb)

补充图S1：经PMA（叠氮溴化丙酯，Propidium Monoazide, PMA）与非PMA处理的样本所获总测序读段占比。
补充图S2：飞行批次层级的微生物相对丰度。不同飞行批次采样的相对丰度结果显示，所有域类群（细菌域、古菌域与真核生物域）均有检出。从飞行1至飞行3，细菌种群丰度呈现显著升高趋势。
补充图S3：国际空间站（International Space Station, ISS）样本微生物组构成的前25种优势物种。将标准化后的测序读段比对至物种级分辨率的参考数据库，该图展示了飞行1至飞行3期间，非PMA与PMA处理样本中各物种的相对丰度对比情况。
补充图S4：经PMA处理的ISS环境样本中，对应微生物门的宏基因组学（Metagenomics）读段丰度。
补充图S5：经PMA处理的ISS环境样本中，对应微生物属的宏基因组学读段丰度。
补充图S6：飞行1、飞行2与飞行3的PMA及非PMA处理样本中，抗菌耐药性（Antimicrobial Resistance, AMR）相关宏基因组学读段的丰度。
补充图S7：与毒力（Virulence）相关的宏基因组学读段丰度。(ZIP 压缩包，大小180 KB)