Transection of the hepatic parenchyma associated or not with the contralateral portal vein branch ligature and its effect in liver regeneration

ABSTRACT Objective To analyze the influence of portal vein ligation in hepatic regeneration by immunohistochemical criteria. Methods Ten pigs divided into two groups of five animals underwent hepatectomy in two stages, and the groups were differentiated by ligation or not of the left portal vein tributary, which is responsible for vascularization of the left lateral and medial lobes of the pig liver. Five days after the procedure, the animals underwent liver biopsies for further analysis of histological and immunohistochemical with marker Ki67. Results The group submitted to hepatectomy with vascular ligation showed an increase of approximately 4% of hepatocytes in regeneration status, as well as a greater presence of Kupffer and inflammatory cells as compared to control. Conclusion As a result of positive cell replication observed through the Ki67 marker, we can suspect that the ligation of a tributary of the portal vein associated with liver resection promoted a greater stimulus of liver regeneration when compared to liver resection alone.

摘要
研究目的：通过免疫组织化学标准分析门静脉结扎对肝脏再生的影响。
方法：将10头猪分为两组，每组5头，实施两步肝切除术；两组的区分依据为是否结扎负责为猪肝脏左外侧叶与左内侧叶提供血管供血的左门静脉分支。术后5天，采集动物肝脏活检样本，用于后续以Ki67标记物开展的组织学与免疫组织化学分析。
结果：相较于对照组，接受伴血管结扎的肝切除术组的处于再生状态的肝细胞占比提升约4%，且库普弗细胞（Kupffer）与炎症细胞的浸润程度显著更高。
结论：基于通过Ki67标记物观测到的阳性细胞增殖结果，我们可推断：相较于单纯肝切除术，结扎门静脉分支联合肝切除术可对肝脏再生产生更强的刺激作用。