Plasma cell dyscrasias expression profiles associated with distinct IGH translocations in multiple myeloma

This series of microarray experiments contains the gene expression profiles of purified plasma cells (PCs) obtained from 7 monoclonal gammopathy of undetermined significance (MGUS), 39 newly diagnosed multiple myeloma (MM) and 6 plasma-cell leukaemia (PCL) patients. PCs were purified from bone marrow Seriess, after red blood cell lysis with 0.86% ammonium chloride, using CD138 immunomagnetic microbeads. The purity of the positively selected PCs was assessed by morphology and flow cytometry and was > 90% in all cases. 5 micrograms of total RNA was processed and hybridized to the Affymetrix HG-U133A chip following the manufacturer's instructions. After scanning, the images were processed using Affymetrix MicroArray Suite (MAS) 5.0 software to generate gene expression intensity values. Arrays normalization was performed using MAS 5.0 "global scaling" procedure, which normalizes the signals of different experiments to the same target intensity (TGT=100). Keywords: other

本系列微阵列实验包含了纯化血浆细胞（plasma cells, PCs）的基因表达谱，这些血浆细胞取自7例意义未明单克隆丙种球蛋白病（monoclonal gammopathy of undetermined significance, MGUS）患者、39例初诊多发性骨髓瘤（multiple myeloma, MM）患者以及6例浆细胞白血病（plasma-cell leukaemia, PCL）患者。血浆细胞从骨髓样本中纯化得到：先以0.86%氯化铵裂解红细胞，再通过CD138免疫磁微珠进行分选。通过形态学与流式细胞术对阳性分选得到的血浆细胞纯度进行评估，所有样本的纯度均大于90%。取5微克总RNA，按照制造商说明书的操作流程处理后，与Affymetrix HG-U133A芯片进行杂交。扫描芯片后，使用Affymetrix MicroArray Suite (MAS) 5.0软件处理图像以生成基因表达强度值。采用MAS 5.0的“全局缩放”（global scaling）流程进行阵列归一化，将不同实验的信号归一化至相同靶强度（TGT=100）。关键词：其他