Gene expression profile at single cell level of stromal cells(SCs) from the periosteum and bone marrow. Gene expression profile at single cell level of stromal cells(SCs) from the periosteum and bone marrow

Stromal cells in bone are considered as a heterogenous population that have different locations and functions, though all of them can self-renew and differentiate into osteoblasts. We used single cell RNA sequencing(scRNA-seq) to dissect the heterogeneity of Stromal cell in the bone. Overall design: Stromal cell in bone marrow and bone periosteum were digested and isolated by Fluorescence-activated cell sorting (FACS). Then cells were analyzed using scRNA-seq.

骨基质细胞（Stromal cells）被认为是一类具有各异组织定位与生物学功能的异质性细胞群体，尽管所有此类细胞均具有自我更新能力，且可分化为成骨细胞。本研究采用单细胞RNA测序（single cell RNA sequencing, scRNA-seq）技术解析骨基质细胞的异质性。实验设计：对骨髓与骨膜中的基质细胞进行消化处理，通过荧光激活细胞分选（Fluorescence-activated cell sorting, FACS）分离得到目标细胞，随后对分离获得的细胞开展scRNA-seq分析。