Integrative ATAC-seq and RNA-seq Analyses of IPEC-J2 Cells Reveals Porcine Transcription and Chromatin Accessibility Changes Associated with Escherichia coli F18ac Inhibited by Lactobacillus reuteri

<em>Escherichia coli </em>(<em>E. coli</em>) is the main cause of postweaning diarrhea in pigs, leading to economic loss. As a probiotic, <em>Lactobacillus reuteri </em>(<em>L. reuteri</em>) has been used to inhibit<em> E. coli</em> in clinical applications; however, its integrative interactions with hosts remain unclear, especially in pigs. Here, we found that <em>L. reuteri</em> effectively inhibited <em>E. coli </em>F18ac adhering to porcine IPEC-J2 cells, and explored the genome-wide transcription and chromatin accessibility landscapes of IPEC-J2 cells by RNA-seq and ATAC-seq<em>.</em>

大肠杆菌（Escherichia coli，E. coli）是引发猪断奶后腹泻的主要致病菌，可造成显著经济损失。作为一种益生菌，罗伊氏乳杆菌（Lactobacillus reuteri，L. reuteri）在临床应用中已被用于抑制大肠杆菌，但罗伊氏乳杆菌与宿主的整体互作机制仍未明确，尤其在猪宿主中。本研究发现，罗伊氏乳杆菌可有效抑制大肠杆菌F18ac黏附至猪IPEC-J2细胞，并通过RNA测序（RNA-seq）与转座酶可及性测序（ATAC-seq），解析了IPEC-J2细胞的全基因组转录组与染色质可及性景观。