Microfluidic-based mini-metagenomic sample preparation and sequencing of hot spring bacterial communities from the Yellowstone National Park. hot springs metagenome

Microfluidic-based mini-metagenomics is a pipeline for processing and analyzing microbial communities from environmental samples, where each sample is divided into a number of sub-samples with lower biological complexity. The current project uses the microfluidic-based mini-metagenomic approach to analyze a number of hot spring biosamples collected from the Yellowstone National Park, from which genomes representing novel lineages are identified. The associated SRAs refer to raw sequencing reads from sub-samples derived from Yellowstone hot spring microbial communities.

基于微流控的微型宏基因组学（microfluidic-based mini-metagenomics）是一套用于处理与分析环境样本微生物群落的分析流程，该流程可将每份原始样本拆分为若干生物学复杂度更低的子样本。本研究项目采用该基于微流控的微型宏基因组学方法，对采集自黄石国家公园的多份温泉生物样本展开分析，成功从中鉴定出代表全新演化支的基因组序列。相关的SRA（Sequence Read Archive）数据集对应了源自黄石国家公园温泉微生物群落各子样本的原始测序读段。