Single-Cell Transcriptomic Profiling of the Mouse Testicular Germ Cells Reveals Important Role of Phosphorylated GRTH/DDX25 in Round Spermatid Differentiation and Acrosome Biogenesis during Spermiogenesis. Single-Cell Transcriptomic Profiling of the Mouse Testicular Germ Cells Reveals Important Role of Phosphorylated GRTH/DDX25 in Round Spermatid Differentiation and Acrosome Biogenesis during Spermiogenesis

We used single cell RNA sequencing (scRNA-seq) to analyze the gene expression dynamics of germ cell development during spermatogenesis in the testis. Overall design: Testicular cells were isolated from mouse seminferous tubules using 2 step enzymatic digestion followed by filteration using 100 µm and 40 µm cell strainer and analyzed using scRNAseq.

本研究采用单细胞RNA测序（single cell RNA sequencing, scRNA-seq）技术，解析小鼠睾丸精子发生过程中生殖细胞发育的基因表达动态变化。实验设计概述：从小鼠生精小管中分离睾丸细胞，先通过两步酶解法完成细胞消化，随后使用100 µm与40 µm孔径的细胞筛进行过滤富集，最终采用scRNA-seq技术完成测序分析。