Autoimmunity associated allele of tyrosine phosphatase gene PTPN22 enhances anti-viral immunity. Autoimmunity associated allele of tyrosine phosphatase gene PTPN22 enhances anti-viral immunity

In this study, we examine gene expression and function of immune cell subsets to demonstrate how a common allelic variant of PTPN22, which strongly increases the risk of autoimmune disease, promotes successful clearance of an otherwise chronic viral infection. Overall design: At 8 days post infection, splenocytes from 4-5 age matched, sex matched PEP-WT Wild Type and PEP-619WW Mutant mice were removed, processed , and resuspended in a single cell suspension. Splenocytes from each genotype were pooled and FACS sorted for Live CD45+ cells. Sorted cells were analyzed using single cell RNA sequencing via 10x Genomics platform

本研究针对免疫细胞亚群的基因表达与功能开展系统分析，旨在阐明一种可显著升高自身免疫疾病发病风险的常见PTPN22等位基因变异，如何促进原本会进展为慢性的病毒感染被成功清除。
总体实验设计：
感染后第8天，从4-5只年龄、性别匹配的PEP-WT野生型（Wild Type）与PEP-619WW突变型小鼠体内摘取脾细胞，经处理后重悬为单细胞悬液。将各基因型小鼠的脾细胞混合后，通过荧光激活细胞分选（FACS）分选出活的CD45阳性细胞。分选得到的细胞通过10x Genomics平台完成单细胞RNA测序（single cell RNA sequencing）分析。