Optimization of RNP-CRISPR for High-Efficiency Gene Editing in Mouse Intestinal Organoids

This project presents the Sanger sequencing data of the edited Apc and Kiss1 loci in mouse intestinal organoids following CRISPR/Cas9 RNP electroporation. Mouse intestinal organoids serve as valuable models for studying intestinal development, homeostasis, and diseases like colorectal cancer. The CRISPR/Cas9 ribonucleoprotein (RNP) electroporation technique was optimized to achieve high-efficiency gene editing with minimal cellular toxicity, enabling precise genetic modifications without compromising the organoids' functionality. The Sanger sequencing data provided here offers insights into the accuracy and efficiency of the gene editing process at the Apc and Kiss1 sites, facilitating further research into the mechanisms of intestinal diseases and the development of potential therapeutic strategies.

本数据集展示了经CRISPR/Cas9核糖核蛋白（RNP）电穿孔处理后，小鼠肠道类器官（mouse intestinal organoids）中Apc与Kiss1基因位点编辑后的桑格测序（Sanger sequencing）数据。小鼠肠道类器官是研究肠道发育、内稳态以及结直肠癌等肠道疾病的极具价值的实验模型。本研究优化了CRISPR/Cas9核糖核蛋白（RNP）电穿孔技术，可在实现高效基因编辑的同时将细胞毒性降至最低，能够在不损害类器官功能的前提下完成精准的遗传修饰。本数据集提供的桑格测序数据可揭示Apc与Kiss1位点基因编辑过程的准确性与效率，有助于推动肠道疾病致病机制研究及潜在治疗策略的开发。