Surface Proteome of Plasma Extracellular Vesicles differentiates between SARS-CoV-2 and Influenza Infection

Introduction: Small extracellular vesicles (sEVs) play a role in the pathophysiology of viral respiratory infections, and may be suitable biomarkers for COVID-19 and Influenza infections or targets for treatment. We investigated differences in the surface proteome of plasma sEVs in patients with COVID-19 and Influenza.Methods: In a discovery cohort with 117 patients, we used a random forest (RF) classifier in order to discriminate COVID-19 and Influenza patients based on routine clinical parameters. Furthermore, plasma samples from these patients were analysed with an EV Array containing 33 antibodies to capture sEVs, which were then visualized with a combination of CD9, CD63, and CD81 antibodies引言：小细胞外囊泡（small extracellular vesicles, sEVs）在病毒性呼吸道感染的病理生理过程中发挥重要作用，有望成为新型冠状病毒肺炎（COVID-19）与流感感染的潜在生物标志物或治疗靶点。本研究旨在探究新型冠状病毒肺炎与流感患者血浆来源小细胞外囊泡的表面蛋白质组差异。 方法：在包含117名患者的发现队列中，本研究采用随机森林（random forest, RF）分类器，基于常规临床参数对新型冠状病毒肺炎与流感患者进行分类鉴别。此外，本研究使用搭载33种抗体的细胞外囊泡阵列（EV Array）对上述患者的血浆样本进行分析以捕获小细胞外囊泡，随后通过CD9、CD63及CD81抗体组合对其进行可视化检测。