Data for: Modulation of the transcriptomic profile of the R2C tumor Leydig cell line by the adipose tissue derived hormone leptin

We examined if leptin could alter the transcriptome of the constitutively steroidogenic rat tumor Leydig cell line R2C. These cells were treated with high levels leptin (1000 ng/ml) for 4 h, followed by mRNA extraction and RNA-Seq analysis. Total RNA extractions from cultured R2C Leydig cells were performed using the E.Z.N.A. Total RNA kit (Omega Bio-Tek, Inc., Norcross, GA). Libraries were constructed using the TruSeq Stranded mRNA LT (Illumina) for cDNA synthesis and polyA enrichment. Quality and quantification of libraries were assessed using the Agilent 2100 Bioanalyzer (Agilent Technologies) and KAPA SYBR® FAST qPCR Kit (KAPA Bio Systems), respectively. Transcriptomic sequencing was performed on an Illumina NextSeq 500 in paired reads layout with approximately 13 million reads per sample and 75 bp of sequencing length. Libraries and next generation sequencing were completed by Applied Biological Materials Inc. (Richmond, BC, CAN). Paired-end raw reads are available in FASTQ format.

本研究旨在探究瘦素（leptin）是否能够改变组成性类固醇生成大鼠肿瘤睾丸间质细胞系R2C的转录组。将上述细胞以高剂量瘦素（1000 ng/ml）处理4小时，随后进行mRNA提取与RNA测序（RNA-Seq）分析。本研究采用E.Z.N.A.总RNA提取试剂盒（Omega Bio-Tek公司，美国佐治亚州诺克罗斯市）对培养的R2C睾丸间质细胞进行总RNA提取。采用Illumina公司的TruSeq Stranded mRNA LT建库试剂盒进行cDNA合成与polyA富集，以构建测序文库。分别采用安捷伦2100生物分析仪（Agilent Technologies公司）与KAPA SYBR® FAST荧光定量PCR试剂盒（KAPA生物系统公司）对文库的质量与浓度进行评估。转录组测序在Illumina NextSeq 500平台上完成，采用配对末端读长模式，每个样本约产生1300万条测序读段，测序读长为75 bp。所有文库构建与下一代测序工作均由加拿大不列颠哥伦比亚省里士满市的Applied Biological Materials公司完成。配对末端原始测序读段以FASTQ格式公开可用。