Expression data from human Circulating Tumor Cells (CTC) and HT-29 cells. Homo sapiens

Unraveling the yet unknown molecular mechanisms regulating the biology of metastasis-competent Circulating Tumor Cells (CTCs) is important for better understanding metastatic growth and disease relapses in patients with colon cancer. We investigated and compared the transcriptome profiles of the CTC line and of a cell line derived from a primary colon cancer to get some insight into the specific molecular mechanism of metastasis-competent CTCs. We used microarrays to establish the molecular portrait of the metastasis-competent CTC and of HT-29 cells Overall design: CTC line was isolated from the blood of a patient with colon cancer and the HT-29 line was derived from a primary colon cancer. Affymetrix Human Genome U133 Plus 2.0 DNA chips were performed to analyze the transcriptomes of CTC and HT29. The Affymetrix 3’ IVT express protocol was used to prepare cRNA (one-cycle amplification). The gene expression data were extracted using Affymetrix Expression Console software.

阐明调控具有转移潜能的循环肿瘤细胞（Circulating Tumor Cells, CTCs）生物学特性的未知分子机制，对于深入理解结肠癌患者的转移灶生长及疾病复发机制具有重要意义。本研究通过对比分析循环肿瘤细胞系与原发性结肠癌来源细胞系的转录组谱，以期解析具有转移潜能的循环肿瘤细胞的特异性分子机制。本研究采用基因芯片技术构建具有转移潜能的循环肿瘤细胞与HT-29细胞的分子表达谱。实验设计概况：循环肿瘤细胞系分离自一名结肠癌患者的血液样本，HT-29细胞系来源于原发性结肠癌组织；采用Affymetrix Human Genome U133 Plus 2.0 DNA芯片对循环肿瘤细胞与HT29细胞的转录组进行分析。使用Affymetrix 3’ IVT快速实验方案完成cRNA制备（单轮扩增），基因表达数据通过Affymetrix Expression Console软件提取。