Gene expression data from JUNB depleted exponentially growing U2OS-derived UTA6 cells

To address the role of JUNB in cell proliferation, we investigated the consequences of JUNB depletion using U2OS human bone osteosarcoma cells. We used microarrays to detail the programme of JUNB dependent gene expression underlying cell proliferation and identified distinct classes of up and down-regulated genes during this process. Exponentially growing U2OS-derived UTA6 cells were transfected with siJUNB or siControl for 72 hours before RNA preparation and probing of Affymetrix GeneChip Human Transcriptome 2.0 Arrays.

为阐明JUNB基因在细胞增殖中的生物学功能，我们以人类骨肉瘤U2OS细胞系为模型，探究了JUNB基因敲低所引发的细胞效应。我们通过基因芯片技术，系统解析了介导细胞增殖的JUNB依赖性基因表达调控程序，并鉴定出该过程中显著上调与下调的多类基因。将处于指数生长期的U2OS来源UTA6细胞，采用靶向JUNB的小干扰RNA（siJUNB）或阴性对照小干扰RNA（siControl）转染培养72小时后，提取总RNA，随后利用Affymetrix GeneChip人类转录组2.0芯片进行杂交检测。