Gill transcriptome sequencing and de novo annotation of Acanthogobius ommaturus in response to salinity stress

Acanthogobius ommaturus is a euryhaline fish widely distributed in coastal, bay and estuarine areas, showing a strong tolerance to salinity. In order to understand the mechanism of adaptation to salinity stress, RNA-seq was used to compare the transcriptome responses of A. ommaturus to the changes of salinity. Four salinity gradients, 0 psu, 15 psu (control), 30 psu and 45 psu were set to conduct the experiment.矛尾虾虎鱼（Acanthogobius ommaturus）为广盐性鱼类，广泛分布于沿海、海湾及河口区域，对盐度具有极强耐受能力。为阐明其适应盐度胁迫的分子机制，本研究采用RNA测序（RNA-seq）技术，对比分析矛尾虾虎鱼在盐度变化下的转录组应答特征。实验设置了0 psu、15 psu（对照组）、30 psu及45 psu共4个盐度梯度。