Putative mitosomal proteins classified by predicted function: Iron-sulfur cluster assembly, chaperones, redox mechanism and protein translocation and processing.

Mascot score, Mascot total ion score for the identified protein. Coverage, number of unique peptides per identified protein. MiD, mitosomal distribution. Proteins are marked “Y” if their distributions in fractions #3 and #4 of the Optiprep gradient (measured by the iTRAQ ratio) were within the range between Cpn10 and IscU and the window that extended in both directions by half of the distance between these markers. Proteins with ratios outside of this range are indicated with “N”. TargetP and PsortII were used to predict the subcellular location of Giardia proteins. S, secretory; N, non-secretory; M, mitochondrial; O, other. Exp. ver., experimental verification of protein localization using the pONDRA expression vector. The recombinant tagged proteins were localized by fluorescence microscopy. M, mitosome; ER, endoplasmic reticulum; O, other; ? inconclusive. MEMSAT3 and TMHMM were used to predict transmembrane domains. SGP, predicted soluble proteins are marked with number sign (#). Asterisk (*) is used where no data were available. (**) transformed Giardia did not express the recombinant tagged protein.

Mascot评分（Mascot score）：针对鉴定所得蛋白质的Mascot总离子评分。覆盖度：单个鉴定蛋白质所匹配的唯一肽段数量。MiD：纺锤剩体分布（mitosomal distribution）。若某蛋白质在Optiprep密度梯度离心的第3、4组分中的分布（以iTRAQ比值衡量）处于Cpn10与IscU之间的区间，且该区间向两侧延伸至两个标记物间距一半的范围，则将该蛋白质标记为“Y”；比值超出该范围的蛋白质则标记为“N”。采用TargetP和PsortII（TargetP and PsortII）预测贾第虫蛋白质的亚细胞定位，其中S代表分泌型、N代表非分泌型、M代表线粒体定位型、O代表其他类型。Exp. ver.：采用pONDRA表达载体（pONDRA expression vector）开展的蛋白质定位实验验证。通过荧光显微镜对带有标签的重组蛋白质进行定位检测，其中M代表纺锤剩体（mitosome）、ER代表内质网（endoplasmic reticulum）、O代表其他类型，“?”代表结果不确定。采用MEMSAT3和TMHMM（MEMSAT3 and TMHMM）预测蛋白质的跨膜结构域。SGP：预测得到的可溶性蛋白质以井号（#）标注。无可用数据时使用星号（*）标注。（**）表示转化后的贾第虫未表达带有标签的重组蛋白质。