CIL:49759, Lesional skin. In Cell Image Library

Two mm skin were fixed in 2% glutaraldehyde in 0.1 M sodium cacodylate buffer pH 7.4 washed in 0.1 M sodium cacodylate buffer and postfixed in 1% osmiumtetroxide and 1.5% potassiumferrocyanide. Samples were dehydrated, embedded in epon, and sectioned. Ultrathin sections (70 nm) were positioned on single slot holders A600 Nickel supported by Formvar and contrasted with 2% uranylacetate (methanol) followed by Reynolds lead citrate. Acquisition was performed using a Zeiss supra 55 EM (Oberkochen, Germany) with ATLAS software developed by Fibics (Ottawa, Ontario, Canada). Samples were recorded at 2.5 nm pixel size. Scans were stitched, and raw data sets were rendered as HTML files using ATLAS VE viewer software. data sets are orientated such that the epidermis/superficial layers are facing up.

将2mm大小的皮肤组织固定于pH 7.4的0.1 M二甲胂酸钠（sodium cacodylate）缓冲液配制的2%戊二醛溶液中，随后用0.1 M二甲胂酸钠缓冲液漂洗，再经1%四氧化锇（osmiumtetroxide）与1.5%亚铁氰化钾（potassiumferrocyanide）进行后固定。对样品进行脱水处理，以环氧树脂（epon）包埋后切片。将70 nm超薄切片置于覆有Formvar支撑膜的单槽A600镍载网上，先用2%醋酸铀（uranylacetate，甲醇体系）进行染色，再经Reynolds柠檬酸铅染色。图像采集使用德国蔡司（Zeiss）supra 55型电子显微镜（EM），搭配加拿大安大略省渥太华市Fibics公司开发的ATLAS软件完成。采集时像素尺寸设为2.5 nm。对扫描图像进行拼接后，采用ATLAS VE Viewer软件将原始数据集渲染为HTML格式文件。所有数据集均以表皮/表层朝上的方位进行排布。