Evolution of gene expression levels in Anopheles male reproductive organs

Gene expression was determined for Male accessory gland and testes in different members of the Anopheles gambiae complex, including An. gambiae (Savannah, MRA-762), An. coluzzii (Mopti) [2], An. arabiensis [3], An. merus [4] and An. quadriannalatus [5. All the mosquito stocks were obtained from the Malaria Research and Reference Reagent Resource Center (MR4) in Atlanta (https://www.beiresources.org/Catalog/BEIVectors/MRA-762.aspx). References: 1. MR4 wild stocks information. Access Jenuary 2018. (Kisumu) https://www.beiresources.org/Catalog/BEIVectors/MRA-762.aspx 2. MR4 wild stocks information. Access Jenuary 2018. (Mopti) https://www.beiresources.org/Catalog/BEIVectors/MRA-763.aspx 3. MR4 wild stocks information. Access Jenuary 2018. (arabiensis) https://www.beiresources.org/Catalog/BEIVectors/MRA-856.aspx 4. MR4 wild stocks information. Access Jenuary 2018. (merus) https://www.beiresources.org/Catalog/BEIVectors/MRA-1156.aspx 5. MR4 wild stocks information. Access Jenuary 2018. (quadriannulatus) https://www.beiresources.org/Catalog/BEIVectors/MRA-1155.aspx Overall design: MAGs and Testes mRNA profiles of Anopheles species were generated by deep sequencing, in triplicate, using Illumina HiSeq 1500本数据集针对冈比亚按蚊复合体（Anopheles gambiae complex）不同成员的雄性附腺与睾丸开展基因表达量检测，涉及物种包括冈比亚按蚊（萨凡纳株，MRA-762）、科鲁兹按蚊（莫普提株）[2]、阿拉伯按蚊[3]、咸水按蚊[4]以及四按按蚊[5]。所有蚊株均购自亚特兰大疟疾研究与参考试剂资源中心（Malaria Research and Reference Reagent Resource Center, MR4），相关获取链接为https://www.beiresources.org/Catalog/BEIVectors/MRA-762.aspx。 参考文献： 1. MR4野生蚊株信息。2018年1月访问（基苏木株）：https://www.beiresources.org/Catalog/BEIVectors/MRA-762.aspx 2. MR4野生蚊株信息。2018年1月访问（莫普提株）：https://www.beiresources.org/Catalog/BEIVectors/MRA-763.aspx 3. MR4野生蚊株信息。2018年1月访问（阿拉伯按蚊株）：https://www.beiresources.org/Catalog/BEIVectors/MRA-856.aspx 4. MR4野生蚊株信息。2018年1月访问（咸水按蚊株）：https://www.beiresources.org/Catalog/BEIVectors/MRA-1156.aspx 5. MR4野生蚊株信息。2018年1月访问（四按按蚊株）：https://www.beiresources.org/Catalog/BEIVectors/MRA-1155.aspx 实验设计：通过Illumina HiSeq 1500平台进行深度测序，设置三次生物学重复，获取上述按蚊物种的雄性附腺与睾丸mRNA转录组图谱。