Analysis of differentiatng monocytes transcriptome changes in response to type I IFNs

To analyze the transcriptome changes of differentiating monocytes in response to type I IFNs, RNA seq was performed. The MACS-purified mouse BM monocytes were cultured in the presence or absence of I IFNs for 12 h or 24h under the condition of M-CSF, samples were harvested for total RNAs and subjected to RNA sequencing analyses. MACS-purified monocytes from mouse bone marrow were treated with 20 ng/mL M-CSF, together with or without 100U mouse IFNβ for 12h, or 24h. each sample had three replicates. Total RNA was harvested at the indicated time, and then the RNA samples were subjected to RNA sequencing analyses.

为分析分化中的单核细胞响应I型干扰素（type I IFNs）时的转录组变化，本研究开展了RNA测序（RNA-seq）。经磁珠激活细胞分选（MACS）纯化的小鼠骨髓单核细胞，在巨噬细胞集落刺激因子（M-CSF）培养条件下，分别于添加或不添加I型干扰素的培养基中培养12小时或24小时，随后收集样本提取总RNA并进行RNA测序分析。经MACS纯化的小鼠骨髓单核细胞以20 ng/mL的M-CSF培养，同时添加或不添加100U小鼠干扰素β（IFNβ），分别处理12小时或24小时，每组样本设置3次生物学重复。于指定时间点收集总RNA，随后将RNA样本进行RNA测序分析。