<p>Pathological Fraction.</p>

Objectives This study aimed to determine reference intervals (RI) for thyroid hormones based on thyroid antibody levels using different autoanalyzer kits. Methods RI for Thyroid-stimulating hormone (TSH), free thyroxine (fT4) and free triiodothyronine (fT3) were determined according to thyroid antibody levels and independently of thyroid antibody levels using the R statistical program and RefineR algorithm. Results Significant differences in RIs were found between antibody-positive (Ab(+)) and antibody-negative (Ab(–)) individuals. TSH RI varied most notably in Abbott and Siemens analyzers. In females, Abbott showed higher TSH RIs in the Ab(+) group (0.41–7.44 mU/L) than in Ab(–) (0.24–3.50 mU/L). In males, Roche and Beckman exhibited the greatest differences (Roche Ab(+): 0.19–5.77; Ab(–): 0.44–3.63; Beckman Ab(+): 0.12–5.23; Ab(–): 0.39–3.96 mU/L). For fT4, Roche showed increased RIs in females with Ab(+) status (11.42–20.42 vs. 10.34–19.35 pmol/L). In males, Beckman and Siemens autoanalyzers also indicated notable differences. Conclusion Antibody status significantly affects thyroid hormone RI, particularly for TSH. These findings highlight the need for antibody-specific RI and further standardization in establishing reference intervals.研究目的 本研究旨在基于甲状腺抗体水平，结合不同自动分析仪试剂盒，确定甲状腺激素的参考区间（reference intervals, RI）。 研究方法 本研究采用R统计软件与RefineR算法，分别依据甲状腺抗体水平、不依据甲状腺抗体水平，对促甲状腺激素（Thyroid-stimulating hormone, TSH）、游离甲状腺素（free thyroxine, fT4）及游离三碘甲状腺原氨酸（free triiodothyronine, fT3）的参考区间进行确定。 研究结果 本研究发现，抗体阳性（Ab(+)）与抗体阴性（Ab(–)）个体的甲状腺激素参考区间存在显著差异。其中，促甲状腺激素参考区间的差异在雅培（Abbott）及西门子（Siemens）分析仪中最为突出。在女性群体中，雅培分析仪的抗体阳性组促甲状腺激素参考区间为0.41~7.44 mU/L，高于抗体阴性组的0.24~3.50 mU/L。在男性群体中，罗氏（Roche）与贝克曼（Beckman）分析仪的差异最为显著：罗氏抗体阳性组参考区间为0.19~5.77 mU/L，抗体阴性组为0.44~3.63 mU/L；贝克曼抗体阳性组参考区间为0.12~5.23 mU/L，抗体阴性组为0.39~3.96 mU/L。对于游离甲状腺素，罗氏分析仪在抗体阳性的女性群体中表现出更高的参考区间（11.42~20.42 pmol/L 对比 10.34~19.35 pmol/L）。在男性群体中，贝克曼与西门子自动分析仪也呈现出显著的差异。 研究结论 甲状腺抗体状态会显著影响甲状腺激素的参考区间，尤其对促甲状腺激素的影响更为明显。本研究结果提示，需建立针对抗体状态的特异性参考区间，并在参考区间的建立流程中进一步推进标准化工作。