Mapping of ribosome footprints from Arabidopsis thaliana (thale cress)

Translation elongation in Arabidopsis thaliana was studied using ribosome profiling. 3 biological replicates of young, immature leaves of Arabidopsis thaliana, Col-0, grown in short day conditions for 7 weeks were harvested. Ribosomal footprints were produced by digestion with MNase, the ribosomes were purified by centrifugation through a sucrose cushion and the RNA was isolated from the ribosome fraction. The 16-42 nt fraction was sequenced using Illumina's HiSeq 4000.

本研究利用核糖体谱（ribosome profiling）技术，针对拟南芥（Arabidopsis thaliana）的翻译延伸过程展开研究。采集在短日照条件下培养7周的拟南芥Col-0生态型幼嫩未成熟叶片，设置3次生物学重复样本。通过微球菌核酸酶（MNase）酶解制备核糖体保护片段，经蔗糖垫超速离心纯化核糖体后，从核糖体组分中分离RNA。随后选取16~42 nt的RNA片段，采用Illumina HiSeq 4000测序平台进行测序。