Using single-cell RNA-Seq for unbiased analysis of developmental hierarchies (Bulk RNA-Seq). Mus musculus

Single cell RNA seq and bioinformatic analysis is used to characterize myeloid differentiation to uncover novel transcriptional networks and key drivers of hematopoietic development Overall design: Single-cell RNA Seq of 4 different hematopoietic populations, integrated with ChIP Seq involving 4 different markers

本研究采用单细胞RNA测序（single cell RNA seq）与生物信息学分析手段对髓系分化过程进行表征，旨在揭示造血发育中的新型转录网络与关键驱动因子。整体实验设计：对4种不同的造血群体开展单细胞RNA测序，并结合涉及4种不同标记物的染色质免疫沉淀测序（ChIP Seq）分析。