PARSIFAL gut metagenomic sequencing data (Swedish population)

To better understand the lifestyle and environmental drivers of the microbiome and resistome we analyzed 74 stool samples from the cross-sectional PARSIFAL study (https://doi.org/10.1111/j.1398-9995.2005.00939.x) using paired-end shotgun metagenomic sequencing. The study population consisted of children aged 5-13 years living in the Uppsala and Järna regions (Sweden) and entailed participants living on farms, children with anthroposophic lifestyles and two reference groups of children living in the same areas but not following these lifestyles. For this study, all 74 fecal samples of the Swedish part of the PARSIFAL study were obtained. Samples were taken by the parents, first stored at –20 °C, and finally stored at –80 °C before DNA extraction, as described by Dicksved et al. (https://doi.org/10.1128/AEM.02223-06). The participant data and samples that were used for the analyses in this study were collected between October 2000 and May 2002. A questionnaire was completed by the parents and has previously been reported in detail by Alfvén et al. (https://doi.org/10.1111/j.1398-9995.2005.00939.x).

The dataset consists of 170 files with a total size of approximately 504 GB.

为更好地阐明微生物组（microbiome）与耐药组（resistome）的生活方式及环境驱动因子，我们对横断面研究PARSIFAL（https://doi.org/10.1111/j.1398-9995.2005.00939.x）中的74份粪便样本开展双端鸟枪宏基因组测序（paired-end shotgun metagenomic sequencing）分析。该研究的受试人群为居住在瑞典乌普萨拉（Uppsala）与耶尔纳（Järna）地区的5~13岁儿童，涵盖农场居住儿童、遵循人智学生活方式的儿童，以及两个居住于同一区域但未遵循此类生活方式的儿童对照组。本研究采用了PARSIFAL研究瑞典分支的全部74份粪便样本。样本由受试者家长采集，先于-20℃条件下保存，最终在DNA提取前以-80℃冻存，具体操作流程详见Dicksved等人的研究（https://doi.org/10.1128/AEM.02223-06）。本研究所用的受试者数据与样本采集于2000年10月至2002年5月期间。家长填写的调查问卷相关内容，此前已由Alfvén等人详细发表（https://doi.org/10.1111/j.1398-9995.2005.00939.x）。

本数据集共包含170个文件，总容量约为504 GB。