MOESM2 of Initial high-resolution microscopic mapping of active and inactive regulatory sequences proves non-random 3D arrangements in chromatin domain clusters

Additional file 2. Overview of type, sequence location and open chromatin marks of TREs targeted by probe sets used in this study (fosmid pools 1 and 2; 6-kb probes 1 and 2). Data are based on [52]. For the segment covered by 6-kb probe 2, no TREs were identified in the used databases. Sequence coordinates represent the coordinates for hg38 after conversion of hg19 into hg38 by liftover. The used databases do not provide data on ‘open chromatin marks’ H3K9ac and H3K4me3 as additional indirect information for their state of activity at the respective loci in BJ1 cells. In the sheet ‘open chromatin marks,’ available data for IMR90 fibroblasts are shown instead. IMR90 cells show an almost identical DHS[+] profile to BJ1 cells ( www.roadmapepigenomics.org ); accordingly, similar epigenetic signatures between both cell lines can be assumed.

附加文件2：本研究所用探针组（黏粒池1与2；6千碱基探针1与2）靶向的转录调控元件（Transcription Regulatory Elements, TREs）的类型、序列位置及开放染色质标记概况。本研究数据源自文献[52]。对于6千碱基探针2所覆盖的基因组区段，在所使用的数据库中未鉴定到任何转录调控元件。序列坐标为通过LiftOver工具将hg19人类参考基因组坐标转换为hg38版本后的坐标。所用数据库未提供开放染色质标记H3K9ac与H3K4me3的相关数据，无法以此作为BJ1细胞对应位点活性状态的间接辅助信息。在「开放染色质标记」工作表中，取而代之展示的是IMR90成纤维细胞的可用数据。IMR90细胞与BJ1细胞的DNase I超敏位点（DNase I Hypersensitive Site, DHS）谱型几乎完全一致（www.roadmapepigenomics.org）；因此可推定两种细胞系具有相似的表观遗传特征。