Spatial structure imposes sex-specific costs but does not reduce interlocus sexual conflict

Spatial structure is a common feature of all naturally occurring populations. Theoretically, spatial structuring of a habitat could modulate the intensity of interlocus sexual conflict (ISC) in a population, possibly by modulating intersexual encounter rate. We tested this theory using laboratory populations of Drosophila melanogaster by measuring male-induced decline in female fitness in three-patch habitat systems with two alternative habitat types: structured–interconnected and unstructured. Our results on reproductive and survival costs in females suggested significant costs attributable to male presence (i.e. ISC) and to living on structured habitat. However, there was only weak evidence supporting the theory of modulation of ISC by habitat structuring only. Through a follow-up experiment, we also showed that the effect of habitat on ISC is robust to the alteration of female conditions. Therefore, it appears that spatial structuring per se is unlikely to modulate ISC but can impose..., Two randomized block design experiments were conducted on laboratory adapted populations of Drosophila melanogaster. The details of these populations were reported first in Nandy et al. 2016 (https://doi.org/10.1016/j.anbehav.2016.01.019). Laboratory structured habitat was set up by using interconnected plastic vials (for three-patch setups) or one vial and one bottle (for two-patch setups). All traits reported in this dataset were measured in the laboratory - close to the populations' usual natural holding condition (i.e., the laboratory conditions in which they are usually maintained). For full experimental details, see the pre-print - https://www.biorxiv.org/content/10.1101/2022.10.29.514349v3. , , # Data from: Spatial structure imposes sex-specific costs but does not reduce interlocus sexual conflict

**Key to reading the data file:**

Data from all the experiments reported in the manuscript titled “Spatial structure imposes sex-specific costs but does not reduce interlocus sexual conflict” can be found in the spreadsheet file.

Data of different assays from all the experiments have been separated into different tabs in the spreadsheet. Data are arranged vertically with factors/treatments separated in columns. Columns are clearly labeled. The details of the tabs are following.

Tab - Ex1_Progeny count (**Tab 1**): Data on progeny output (MPO/Mean Progeny Output) under two types of Male exposures – limited exposure (LE), continuous exposure (CE), and two levels of Habitat i.e., Structured and Unstructured is from Experiment 1(Ex 1). ‘BLOCK’ refers to three randomized blocks mentioned as B_2, B_3, B_5. Count data is unit less.

Tab - Ex1_Starvation survival time (**Tab 2**): Data o...

空间结构是所有自然存在种群的普遍特征。从理论层面而言，生境的空间结构可调控种群内位点间性冲突（interlocus sexual conflict, ISC）的强度，其潜在作用路径可能为调节雌雄个体间的相遇频率。我们以黑腹果蝇（Drosophila melanogaster）的实验室种群为研究对象，在设置结构化-连通型与非结构化两种生境类型的三斑块生境系统中，通过测定雄性诱导的雌性适合度下降来验证这一理论。针对雌性繁殖与存活成本的分析结果显示，雄性存在（即位点间性冲突）以及栖息于结构化生境，均会带来显著的适合度成本。然而，仅能获得微弱证据支持“生境结构可调控位点间性冲突”这一理论。通过后续补充实验，我们进一步证实，生境对位点间性冲突的影响不受雌性状态改变的干扰。由此可见，空间结构本身似乎并不会调控位点间性冲突，但可施加……

本研究依托经实验室驯化的黑腹果蝇（Drosophila melanogaster）种群，开展了两组随机区组设计实验。该种群的详细信息首次由Nandy等人于2016年发表（https://doi.org/10.1016/j.anbehav.2016.01.019）。实验室结构化生境通过连通的塑料培养管搭建（对应三斑块设置），或采用单个培养管与单个培养瓶组合搭建（对应两斑块设置）。本数据集收录的所有性状均在实验室条件下测定，尽可能贴合种群日常的实验室饲养环境（即种群常规维持的实验室条件）。完整实验细节请参阅预印本：https://www.biorxiv.org/content/10.1101/2022.10.29.514349v3。

# 数据来源：空间结构引发性别特异性适合度成本，但不会减弱位点间性冲突

**数据文件阅读指南：**

本手稿题为"Spatial structure imposes sex-specific costs but does not reduce interlocus sexual conflict"（即《空间结构引发性别特异性适合度成本，但不会减弱位点间性冲突》），其所报道的所有实验数据均收录于该电子表格文件中。所有实验的不同测定指标数据已被分门别类至电子表格的不同工作表（tab）中。数据以纵向排列，各因子/处理以列分隔，各列均配有清晰的标签。各工作表的详细说明如下。

工作表 - Ex1_Progeny count（**工作表1**）：包含实验1（Ex1）中，两种雄性暴露模式——有限暴露（limited exposure, LE）与持续暴露（continuous exposure, CE），以及两种生境水平（结构化与非结构化）下的后代产出量（MPO/平均后代产出量）数据。此处的"BLOCK"指代三个随机区组，即B_2、B_3、B_5。计数数据无量纲。

工作表 - Ex1_Starvation survival time（**工作表2**）：Data o...