Spatially resolved multiomics of human cardiac niches - Multiome GEX

Using Multiome and previously published sc/snRNA-seq data, we studied eight anatomical regions of the human heart including left and right ventricular free walls (LV and RV), left and right atria (LA and RA), left ventricular apex (AX), interventricular septum (SP), sino-atrial node (SAN) and atrioventricular node (AVN). For the first time, we profile the cells of the human cardiac conduction system, revealing their distinctive repertoire of ion channels, G-protein coupled receptors and cell-cell interactions. We map the identified cells to spatial transcriptomic data to discover cellular niches within the eight regions of the heart.

本研究采用多组学（Multiome）及已发表的单细胞/单细胞核RNA测序（sc/snRNA-seq）数据，针对人类心脏的8个解剖区域开展研究，涵盖左、右心室游离壁（LV、RV），左、右心房（LA、RA），左心室心尖（AX），室间隔（SP），窦房结（SAN）与房室结（AVN）。本研究首次对人类心脏传导系统的细胞进行表征分析，揭示了其独特的离子通道、G蛋白偶联受体及细胞间相互作用的表达谱；同时将鉴定得到的细胞映射至空间转录组数据，以解析心脏8个区域内的细胞生态位。