Investigating the role of Hmo1 in transcription by Pol I in yeast.. Investigating the role of Hmo1 in transcription by Pol I in yeast.

The purpose of this study was to investigate the role of the yeast protein, high mobility group protein (Hmo1), in rRNA synthesis. For over twenty years, Hmo1 has been proposed to be an activator of transcription by Pol I, but its regulatory mechanism remains largely undefined. Therefore, we used native elongating transcript sequencing (NET-seq) to explore the role of this factor in transcription by Pol I. Our NET-seq results demonstrated that in cells lacking Hmo1 (hmo1D), Pol I occupancy was significantly increased across the rDNA template, indicating an increased pause propensity in these cells. These data suggest that Hmo1 is an important transcription elongation factor for Pol I. Overall design: Triplicate NET-seq libraries were generated for wild-type (WT) and hmo1△ yeast strains. Please note that the WT samples (in GSE216460/PRJNA893665) were re-analysed in the current study: GEO accession title SRA accessions Biosample accession ========= ==== ========== ============= GSM6674347 WT yeast, untreated, rep1 SRR22028427/SRX18009711 SAMN31430711 GSM6674348 WT yeast, untreated, rep2 SRR22028426/SRX18009712 SAMN31430710 GSM6674349 WT yeast, untreated, rep3 SRR22028425/SRX18009713 SAMN31430709

本研究旨在探究酵母蛋白高迁移率族蛋白（high mobility group protein, Hmo1）在核糖体RNA（rRNA）合成中的作用。二十余年来，Hmo1一直被认为是RNA聚合酶I（Pol I）介导转录的激活因子，但其调控机制仍未完全阐明。因此，本研究采用原生延伸转录测序（native elongating transcript sequencing, NET-seq）技术，探究该因子在Pol I转录过程中的功能。

本研究的NET-seq结果显示，在缺失Hmo1的酵母细胞（hmo1Δ）中，Pol I在核糖体DNA（rDNA）全模板上的结合占位水平显著升高，表明此类细胞的转录暂停倾向增强。上述数据表明，Hmo1是Pol I重要的转录延伸因子。

整体实验设计：本研究为野生型（WT）酵母菌株与hmo1Δ缺失菌株分别构建了3个生物学重复的NET-seq文库。请注意，本研究对已收录于GSE216460/PRJNA893665中的野生型样本进行了重新分析：

| GEO样本编号 | 样本说明 | SRA编号 | 生物样本编号 |
| --- | --- | --- | --- |
| GSM6674347 | 野生型酵母，未处理，重复1 | SRR22028427/SRX18009711 | SAMN31430711 |
| GSM6674348 | 野生型酵母，未处理，重复2 | SRR22028426/SRX18009712 | SAMN31430710 |
| GSM6674349 | 野生型酵母，未处理，重复3 | SRR22028425/SRX18009713 | SAMN31430709 |