Identification of factors involved in JAK/STAT-dependent cell competition

We report the transcriptional profiles obtained by next-generation sequencing (NGS) of FACS-purified cells from the dpp-domain of wing imaginal discs from mid-third instar Drosophila melanogaster larvae. The goals of this study are to compare NGS-derived transcriptome profiling (RNA-seq) of dpp-domain cells that have elevated JAK/STAT signaling or elevated Myc expression compared to control cells. Overall design: Examination of differentially-regulated genes in two types of wing imaginal disc cells, those with elevated JAK/STAT signaling and those with elevated Myc, compared to controls.

本研究报道了取自黑腹果蝇（Drosophila melanogaster）三龄中期幼虫翅成虫盘dpp结构域、经荧光活化细胞分选（Fluorescence-Activated Cell Sorting, FACS）纯化的细胞的下一代测序（Next-Generation Sequencing, NGS）转录组图谱。
本研究旨在通过NGS获取的转录组测序（RNA-seq）数据，对比dpp结构域中JAK/STAT信号通路激活或Myc表达上调的细胞与对照细胞的转录组特征。
实验设计：相较于对照细胞，对两类翅成虫盘细胞的差异表达基因进行检测——分别为JAK/STAT信号通路激活的细胞与Myc表达上调的细胞。