Imaging dataset 02 for mtFociCounter

This is the second dataset associated with the updated manuscript on mtFociCounter. Images are Spinning Disk Confocal Images of unsorted 3t3 NIH mouse fibroblasts stably expressing <strong>mitochondrially </strong>targeted dsRed, immunofluorescence against dsDNA (mitochondrial <strong>nucleoids</strong>) and AlexaFluor 647, and stained <strong>nuclei </strong>with Hoechst. It contains all data necessary to reproduce the testing of mtFociCounter (no primary control; rho0 control; manual cell segmentation), as described in the manuscript. Samples were processed and imaged in parallel, on the same date, in biological replicates (different date). The dataset contains the 3t3 WT data from the following acquisition dates: no primary antibody (no1) vs. with primary antibody against dsDNA (nucleoids):<br> 20221022_no1 vs. wt<br> 20221025_no1 vs. wt<br> 20221029_no1 vs. wt for 3t3 cells depleted of mtDNA (rho0) vs. normal 3t3 cells with mtDNA (wt):<br> 20221223: sample_1 &amp; sample_2 rho0 vs. sample_3 wt for 3t3 WT cells, same raw-data, manually segmented single cells on two independent days:<br> 20221022 -&gt; segmentation on 20221102 vs. segmentation on 20221103<br> 20221029 -&gt; segmentation on 20221102 vs. segmentation on 20221104 For further details of the exact experimental procedure, please refer to the accompanying manuscript, which will soon be made available on BioRxiv.本数据集为针对mtFociCounter的更新版手稿所关联的第二份数据集。所收录图像为未分选的NIH 3T3小鼠成纤维细胞的转盘共聚焦显微镜图像：这些细胞稳定表达线粒体靶向的dsRed，针对dsDNA（线粒体类核（mitochondrial nucleoids））与AlexaFluor 647完成免疫荧光标记，并用Hoechst对细胞核进行染色。本数据集包含复现mtFociCounter测试所需的全部数据，涵盖无一抗对照、rho0对照以及人工细胞分割，与手稿中描述的实验内容一致。样本于同日同步完成处理与成像，并设置了生物学重复（生物学重复于不同日期开展）。本数据集包含以下成像日期获取的3T3野生型（WT）数据：无一抗组（no1）与靶向dsDNA（类核）的一抗组对应的20221022_no1 vs. wt、20221025_no1 vs. wt、20221029_no1 vs. wt；线粒体DNA缺失的3T3细胞（rho0）与携带正常线粒体DNA的野生型3T3细胞（wt）对应的20221223批次：样本1、样本2（rho0）与样本3（wt）；针对野生型3T3细胞，使用相同原始数据且于两个独立日期完成人工单细胞分割的组别：20221022对应的分割日期为20221102与20221103，20221029对应的分割日期为20221102与20221104。如需了解具体实验流程的更多细节，请参阅随附手稿，该手稿即将于BioRxiv平台发布。