single-cell RNA-sequencing of murine intestine 24 hours following acute p53 activation due to Mdm2-loss (Mdm2FM/- Cag-CreERTg (FMTg)) compared to intestine that retain Mdm2 (Mdm2+/- Cag-CreERTg (Tg))

FMTg (Mdm2FM/- Cag-CreERTg) and Tg (Mdm2+/- Cag-CreERTg) mice were administered 6mg/40g of Tamoxifen dissolved in corn-oil and sacrificed 24 hours after injection. Single cells were isolated from fresh small intestine samples to compare single cell transcriptomes between intestine with high or control p53 activity One FMTg small intestine and one Tg small intestine were isolated from mice 24 hours following a single 6mg/40g tamoxifen injection. Single cells were isolated from fresh tissues and then processed through the 10X genomics platform

将FMTg（Mdm2FM/- Cag-CreERTg）与Tg（Mdm2+/- Cag-CreERTg）小鼠给予溶于玉米油的6mg/40g剂量他莫昔芬（Tamoxifen），于给药后24小时处死。从新鲜小肠样本中分离单细胞，用于对比p53活性升高组与对照组小鼠小肠的单细胞转录组差异。具体实验流程如下：在单次给予6mg/40g他莫昔芬后的24小时，分别从两种基因型小鼠体内各分离一份小肠组织；将新鲜组织中的单细胞分离后，通过10X Genomics平台完成后续处理。