Integrative single-cell and spatial transcriptomic analyses identify a pathogenic cholangiocyte niche and TNFRSF12A as therapeutic target for biliary atresia

We performed single cell 5' RNA sequencing, and B cell receptor and T cell receptor V(D)J sequencing analyses on liver biopsies of nine patients with biliary atresia and three patients with choledochal cyst at diagnosis to generate the liver cell atlas. Single cell 5' RNA sequencing and V(D)J sequencing analyses of liver biopsies from nine patients with biliary atresia and three patients with choledochal cyst were collected at the time of diagnosis. 10x Genomics Chromium Next GEM Single Cell 5' RNA-seq and single cell V(D)J sequencing. **Submitter declares that the raw data have not been provided due to patient privacy concerns**

本研究针对9例确诊胆道闭锁（biliary atresia）患者与3例确诊胆总管囊肿（choledochal cyst）患者的肝脏活检组织，开展单细胞5'端RNA测序以及B细胞受体（B cell receptor）、T细胞受体（T cell receptor）V(D)J测序分析，以此构建肝脏细胞图谱。本数据集采集了确诊时的9例胆道闭锁患者与3例胆总管囊肿患者肝脏活检组织的单细胞5'端RNA测序及V(D)J测序分析数据。本次测序采用10x Genomics Chromium Next GEM单细胞5'端RNA测序与单细胞V(D)J测序平台。**提交者声明：受患者隐私保护相关要求限制，暂未提供原始测序数据**