Transcriptional profiling of zebrafish embryos developmentally exposed to oxygenated PAHs 1,9-benz-10-anthrone and benzanthracene-7,12-dione. Danio rerio

The purpose of this study was to identify transcripts differentially expressed in zebrafish embryos exposed to two oxygenated PAHs, 1,9-benz-10-anthrone and benzanthracene-7,12-dione, which cause abnormal development. Overall design: We used RNA-seq (Illumina HiSeq) to identify mRNA profiles of whole zebrafish embryos exposed to 10 μM 1,9-benz-10-anthrone, benzanthracene-7,12-dione or vehicle control (1% DMSO) from 6-48 hours post fertilization

本研究旨在鉴定暴露于两种可导致发育异常的含氧多环芳烃（oxygenated PAHs）——1,9-苯并-10-蒽酮与苯并蒽-7,12-二酮——后的斑马鱼胚胎中差异表达的转录本。整体实验设计：本研究采用RNA测序（RNA-seq，基于Illumina HiSeq平台），对受精后6~48小时的完整斑马鱼胚胎的mRNA表达谱进行鉴定，实验组分别暴露于10 μM的1,9-苯并-10-蒽酮、苯并蒽-7,12-二酮，对照组以1%二甲基亚砜（DMSO）作为溶剂对照。