Zea mays Transcriptome or Gene expression. Zea mays

Four tissues (leaves, 1 mm anthers, 1.5 mm anthers, pollen) from one hybrid and one inbred maize line were hybridized with the W23 inbred on Agilent oligonucleotide microarrays with 21K elements. Tissue-specific gene expression patterns were documented with leaves having the most tissue-specific transcripts. Haploid pollen expresses about half as many genes as the other samples. High overlap of gene expression was found between leaves and anthers. Anther and pollen transcript expression showed high conservation among the three lines while leaves had more divergence. Antisense transcripts represented about 6 to 14 percent of total transcriptome by tissue type but were similar across lines. Gene Ontology (GO) annotations were assigned and tabulated. Enrichment in GO terms related to cell cycle functions was found for the identified antisense transcripts. Microarray results were validated via quantitative real time PCR and by hybridization to a second oligonucleotide microarray platform. Keywords: genetic background comparison Overall design: We analyzed 4 tissues from 3 maize lines, each with 3 biological replicates, with W23 as a reference, and hybridized to 24 arrays.

本研究选取1个杂交玉米品系与1个自交玉米品系的4种组织（叶片、1 mm花药、1.5 mm花药、花粉），与W23自交系进行杂交，使用搭载21K个探针的安捷伦（Agilent）寡核苷酸微阵列（oligonucleotide microarray）完成实验。本研究记录了组织特异性基因表达模式：叶片的组织特异性转录本数量最多；单倍体花粉的表达基因数量约为其他样本的一半；叶片与花药之间的基因表达重叠度较高。花药和花粉的转录本表达在三个品系间保守性较强，而叶片的表达差异更为显著。不同组织的反义转录本（antisense transcripts）约占总转录组的6%至14%，且在不同品系间的占比相近。本研究已完成基因本体（Gene Ontology, GO）注释的赋值与制表，所鉴定的反义转录本显著富集于与细胞周期功能相关的GO术语。本研究通过实时定量聚合酶链式反应（quantitative real time PCR, qRT-PCR）以及在另一套寡核苷酸微阵列平台上的杂交实验，验证了微阵列结果。关键词：遗传背景比较 实验整体设计：本研究以W23为参照品系，对3个玉米品系的4种组织进行分析，每个品系的每种组织设置3次生物学重复，共计使用24张微阵列芯片进行杂交。