Chromatin Accessibility Reveals Insight into Androgen Receptor Activation and Transcriptional Specificity. Homo sapiens

Using DNase-seq, mRNA-seq and publicly available ChIP-seq data sets, we examined the role of chromatin accessibility (DNase-seq) in androgen receptor binding to the genome (ChIP-seq) and AR-mediated transcriptional changes (mRNA-seq). Our data reveals genome-wide changes in chromatin structure that correspond to AR binding and differential gene expression. A focused examination of DNase-seq data around androgen receptor motifs within androgen receptor ChIP-seq peaks reveals distinct patterns of protection from DNaseI cleavage. Overall design: Examination of chromatin accessibility (DNase-seq), AR binding (AR ChIP-seq), and transcription (mRNA-seq) in LNCaP cells before and after 12 hours of 1 nM R1881 treatment This Series represents the RNA-Seq data only. Exon microarray data generated under the same conditions is available through GSE15805. The DNase-seq data is publicly available through GSE32970 as well as the UCSC genome browser (genome.ucsc.edu) under Regulation::ENCODE DNase/FAIRE::Duke DNaseI HS:LNCaP and LNCaP + Andro. The accession numbers for the ChIP-seq experiments used are GSE14097 and GSE28126.

本研究借助脱氧核糖核酸酶敏感位点测序（DNase-seq）、信使RNA测序（mRNA-seq）以及公开可用的染色质免疫共沉淀测序（ChIP-seq）数据集，探究了染色质可及性（通过DNase-seq检测）在雄激素受体（androgen receptor, AR）结合基因组（通过ChIP-seq检测）以及AR介导的转录调控变化（通过mRNA-seq检测）中所发挥的作用。本研究数据揭示了全基因组范围内染色质结构的变化，这些变化与AR结合以及差异基因表达情况相吻合。对雄激素受体染色质免疫共沉淀测序峰内的雄激素受体基序周边的DNase-seq数据进行聚焦式分析后，可观察到独特的DNase I酶切保护模式。实验整体设计：在以1纳摩尔浓度的R1881处理LNCaP细胞12小时前后，分别检测其染色质可及性（DNase-seq）、AR结合情况（AR ChIP-seq）以及转录水平（mRNA-seq）。本数据集仅包含RNA测序（RNA-seq）数据。在相同实验条件下生成的外显子微阵列数据可通过GSE15805获取。脱氧核糖核酸酶敏感位点测序（DNase-seq）数据可通过GSE32970以及加州大学圣克鲁兹分校基因组浏览器（genome.ucsc.edu）获取，其分类标签为Regulation::ENCODE DNase/FAIRE::Duke DNaseI HS:LNCaP和LNCaP + Andro。本研究所用的染色质免疫共沉淀测序（ChIP-seq）实验数据集的登录号为GSE14097与GSE28126。