Hematological and biochemical profile of BALB/c nude and C57BL/6 SCID female mice after ovarian xenograft

Abstract Hematological and biochemical profile studies help to evaluate functional changes of animals used in experiments. The aim of this study was to determine the hematological and biochemical profile of immunosuppressed BALB/c nude and C57BL/6 SCID mice after bovine ovarian xenotransplantation. Therefore, a total of 74 female mice were divided into four groups: non-xenotransplanted animals, xenotransplanted animals, xenotransplanted animals treated with eCG and xenotransplanted animals treated with FSH + LH. After anesthesia, blood samples were collected and hematologic and biochemical values were evaluated. The results showed no significant differences (p ≤ 0.05) for hematological parameters between the control group and the treatment groups of both strains. However, considering the biochemical profile, it was observed an increase of AST concentrations (p ≤ 0.05) in both strains and a decrease of ALT concentrations (p ≤ 0.05) only in C57BL/6 SCID strain of the groups subjected to hormonal treatment compared with those non subjected. Additionally, the values of the renal enzymes, urea and creatinine, did not differ (p ≤ 0.05) between the groups. Our findings suggest that the xenotransplantation procedure as well as the hormonal dosages had no significant effect on the well-being of the animals considering the evaluated hematological and biochemical profile.

摘要：血液学与生化指标检测可有效评估实验动物的机能变化。本研究旨在探明牛卵巢异种移植后，免疫缺陷型BALB/c裸鼠（BALB/c nude）与C57BL/6 SCID小鼠的血液学与生化指标谱。本研究共纳入74只雌性小鼠，分为4组：非异种移植对照组、异种移植组、异种移植后经孕马血清促性腺激素（equine chorionic gonadotropin, eCG）处理组，以及异种移植后经促卵泡素（follicle-stimulating hormone, FSH）与促黄体素（luteinizing hormone, LH）联合处理组。对小鼠实施麻醉后采集血液样本，检测其血液学与生化指标。结果显示，两种品系小鼠的对照组与各处理组间的血液学参数均无显著差异（p ≤ 0.05）。就生化指标而言，与未接受激素处理的小鼠相比，接受激素处理的两组小鼠中，两种品系的天冬氨酸氨基转移酶（aspartate aminotransferase, AST）浓度均显著升高（p ≤ 0.05）；而丙氨酸氨基转移酶（alanine aminotransferase, ALT）浓度仅在C57BL/6 SCID品系小鼠中出现显著降低（p ≤ 0.05）。此外，肾相关酶、尿素及肌酐的检测值在各组间均无显著差异（p ≤ 0.05）。本研究结果表明，结合本次评估的血液学与生化指标来看，异种移植操作及激素给药方案对实验动物的健康状态无显著不良影响。