Polysome profiling of wild-type and gld-1 mutant animals. Caenorhabditis elegans

To determine the translational status of mRNAs in wild-type and gld-1 mutant animals, we analyzed total and polysomal RNA levels in both strains by tiling arrays Overall design: A total of 16 samples was analyzed (quadruplicates of wild-type_total, wild-type_polysomal, gld-1_total, gld-1_polysomal RNA respectively)

为确定野生型（wild-type）与gld-1突变体（gld-1 mutant）动物体内mRNA的翻译状态，我们采用平铺式微阵列芯片（tiling arrays）对两种品系的总RNA与多聚核糖体RNA（polysomal RNA）水平进行了分析。实验设计概述：本研究共分析16份样本，分别为野生型总RNA、野生型多聚核糖体RNA、gld-1突变体总RNA、gld-1突变体多聚核糖体RNA的四重复样本。