Label-Free Quantitative Proteomic Profiling of LAD2 Mast Cell Releasates

Tween-80 is one of the most important causes resulting in anaphylactoid reaction. However, its mechanism remains unclear. A Label-free LCMS/MS-based proteomics was used to analyze Tween-80-stimulated LAD2 mast cell releasates. Out of a total dataset of 2546 proteins, 882 proteins were found in the supernate samples of Tween-80-treated LAD2 mast cells; 313 proteins were up-expressed and 111 proteins were down-expressed. KEGG pathway analysis showed that endocytosis was the largest class of pathway. A total of 52 proteins were involved in endocytosis, and mainly related to G-protein-coupled receptor (GPCR)-endocytosis and EGFR-endocytosis

吐温-80（Tween-80）是引发类过敏反应的关键诱因之一，但其具体作用机制尚未明确。本研究采用基于无标记液相色谱-串联质谱（Label-free LC-MS/MS）的蛋白质组学技术，对经吐温-80刺激的LAD2肥大细胞分泌释放物进行分析。在总计2546个蛋白质的数据集内，吐温-80处理组的LAD2肥大细胞上清样本中检出882种蛋白质；其中313种蛋白质呈上调表达，111种蛋白质呈下调表达。京都基因与基因组百科全书通路（KEGG通路）富集分析结果显示，内吞作用为富集程度最高的通路类别，共计52种蛋白质参与该通路，且主要与G蛋白偶联受体（GPCR）内吞及表皮生长因子受体（EGFR）内吞相关。