InVERT final results

Primary human bronchial epithelial cells were differentiated at air liquid interface for 3 weeks in Pneumacult media. Infected cells were infected with Cal/07/09 and harvested at 6 and 12 hours post-infection. After harvest, cells were sorted into ciliated and secretory cells via FACS and submitted for stranded RNAseq. Mock infected cells were also included as a control and sorted into ciliated and secretory cells for stranded RNAseq. N=4 replicates were included for each condition.Raw sequencing data has been deposited on NCBI under BioProject number XXXX. RNAseq data was processed with the InVERT pipeline. The final results are included in these files.

原代人支气管上皮细胞于气液界面（air liquid interface, ALI）环境中，使用Pneumacult培养基分化培养3周。感染组细胞以Cal/07/09毒株感染，并分别于感染后6小时、12小时收获细胞。细胞收获后，通过荧光激活细胞分选术（Fluorescence-Activated Cell Sorting, FACS）将其分为纤毛细胞（ciliated cells）与分泌细胞（secretory cells），随后进行链特异性RNA测序（stranded RNAseq）。本研究同时设置假感染对照组，同样将假感染细胞分选为纤毛细胞与分泌细胞并进行链特异性RNA测序。每个实验条件均设置4次生物学重复。原始测序数据已提交至美国国家生物技术信息中心（National Center for Biotechnology Information, NCBI），生物项目编号为XXXX。RNA测序数据通过InVERT分析流程进行处理，最终分析结果已收录于本数据集文件中。