Panama fungal phyllosphere sequences

Fungal LSU sequence data (primers LR0R/LR3) and metadata for fungal communities sampled from plant leaf surfaces on Barro Colorado Island, Panama. Data collection procedures are described in the paper "Plant traits and taxonomy drive host associations in tropical phyllosphere fungal communities" by Steven Kembel and Rebecca Mueller (manuscript in review at Botany). Multiple sets of files are included in this data archive: <strong>fungi_phyllosphere_metadata.txt</strong> - a tab-delimited text file with information on barcode sequences and host species identity for each sample. The columns of the text file include a sample identifier (SampleID), forward and reverse barcode sequences* (ForwardBarcodeSequence and ReverseBarcodeSequence), and the identity of the host plant species from which each sample was collected (HostSpecies). * note that in the raw sequence data, a combinatorial or dual-index barcoding approach was used to uniquely identify samples. The first 6bp of the barcode are on the forward read, and the final 6bp of the barcode are on the reverse read. The barcodes will need to be combined into a single 12bp construct prior to parsing into samples, or parsed with software capable of processing dual-indexed sequences. <strong>fungi1.fastq.tgz.part-xx</strong><br><strong>fungi2.fastq.tgz.part-xx</strong> - raw sequence data in FASTQ format, produced by two sequencing runs using Illumina MiSeq 250bp paired-end sequencing technology. Due to file size limitations, the original tar/gzip archives have been split into multiple parts. To recombine and uncompress the sequence files, the following commands work in a Linux/Unix environment: cat fungi1.fastq.tgz.part-* | tar xz<br>cat fungi2.fastq.tgz.part-* | tar xz

巴拿马巴罗科罗拉多岛植物叶表面采样的真菌群落的真菌LSU（Large Subunit）序列数据（所用引物为LR0R/LR3）及元数据。数据采集流程详见Steven Kembel与Rebecca Mueller投稿于《Botany》的待审论文"Plant traits and taxonomy drive host associations in tropical phyllosphere fungal communities"。本数据存档包含多组文件：**fungi_phyllosphere_metadata.txt**——制表符分隔的文本文件，包含各样本的条形码序列与宿主物种信息。该文本文件的列包括：样本标识符（SampleID）、正向与反向条形码序列（ForwardBarcodeSequence、ReverseBarcodeSequence），以及采集样本的宿主植物物种名称（HostSpecies）。*注：原始序列数据采用组合式双索引条形码方法以唯一区分样本。条形码的前6bp位于正向测序读段，后6bp位于反向测序读段。在进行样本拆分前，需将两段条形码拼接为完整的12bp序列，或使用支持双索引序列处理的软件进行拆分。
**fungi1.fastq.tgz.part-xx**与**fungi2.fastq.tgz.part-xx**——采用Illumina MiSeq 250bp双端测序技术生成的FASTQ格式原始序列数据，分属两次测序运行。由于文件大小限制，原始tar/gzip压缩归档文件已被拆分为多个分卷。在Linux/Unix环境下，可通过以下命令重新合并并解压序列文件：
cat fungi1.fastq.tgz.part-* | tar xz
cat fungi2.fastq.tgz.part-* | tar xz