OMAP-34: Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Human Esophagus with CODEX

OMAP-34 was designed for CODEX imaging of human fresh frozen esophagus samples (https://doi.org/10.1016/j.cell.2018.07.010; https://doi.org/10.1038/s41596-021-00556-8). The panel contains 31 antibodies; β-Catenin (CTNNB1) was used for cell segmentation while Hoechst served as the nuclear marker. This OMAP provides a spatial context for 8 anatomical structures present in the esophagus: basal epithelium, suprabasal epithelium, superficial epithelium, lamina propria, muscularis mucosae, submucosa, muscularis propria, and blood vessels. Within these layers, the panel covers 18 cell types, including basal, suprabasal, and superficial squamous epithelial cells, stromal fibroblasts, endothelial cells, smooth muscle/myofibroblasts, and multiple immune subsets. Several markers in the panel capture disease-related states such as fibrosis and stromal activation, DNA damage and tumor suppression, and cellular stress or apoptosis. Images were acquired using the Akoya Biosciences Phenocycler Fusion system. For visualization purposes, the minimum and maximum intensity ranges of certain markers were adjusted to enhance signal clarity. All images are provided in .tif format and include corresponding metadata files in .csv format.OMAP-34 专为人类新鲜冷冻食管样本的 CODEX 成像（CODEX imaging）实验设计（相关文献：https://doi.org/10.1016/j.cell.2018.07.010；https://doi.org/10.1038/s41596-021-00556-8）。该抗体组合包含31种抗体：β-连环蛋白（β-Catenin, CTNNB1）用于细胞分割，Hoechst 则作为细胞核标记物。本 OMAP 数据集可提供食管内8种解剖结构的空间定位信息，分别为基底上皮、基上层上皮、表层上皮、固有层、黏膜肌层、黏膜下层、固有肌层及血管。在上述组织层中，该抗体组合可覆盖18种细胞类型，包括基底鳞状上皮细胞、基上层鳞状上皮细胞、表层鳞状上皮细胞、基质成纤维细胞、内皮细胞、平滑肌/肌成纤维细胞，以及多种免疫细胞亚群。该组合中的多种标记物可捕获与疾病相关的病理状态，例如纤维化与基质激活、DNA损伤与肿瘤抑制，以及细胞应激或细胞凋亡。 图像通过 Akoya Biosciences 公司的 Phenocycler Fusion 系统采集获取。为优化可视化效果，研究人员已调整部分标记物的强度上下限范围，以提升信号清晰度。所有图像均以 .tif 格式提供，并附带对应的 .csv 格式元数据文件。