The non-coding RNA CcnA modulates the master cell cycle regulators CtrA and GcrA in Caulobacter crescentus

Bacteria are powerful models for understanding how cells divide and accomplish global regulatory programs. In Caulobacter crescentus, a cascade of essential master regulators supervises the correct and sequential activation of DNA replication, cell division and development of different cell types. Among them, the response regulator CtrA plays a crucial role coordinating all those functions. Here, for the first time we describe the role of a novel factor named CcnA, a cell cycle regulated ncRNA located at the origin of replication, presumably activated by CtrA and responsible for the accumulation of CtrA itself. In addition, CcnA may be also involved in the inhibition of translation of the S-phase regulator, GcrA, by interacting with its 5’ untranslated region (5’-UTR). Performing in vitro experiments and mutagenesis, we propose a mechanism of action of CcnA based on liberation (ctrA) or sequestration (gcrA) of their ribosome-binding site (RBS). Finally, its role may be conserved in othe..., , , # Data from: The non-coding RNA CcnA modulates the master cell cycle regulators CtrA and GcrA in Caulobacter crescentus

[https://doi.org/10.5061/dryad.4mw6m909k](https://doi.org/10.5061/dryad.4mw6m909k)

## Description of the data and file structure

Fastq files included. See `metadata.txt` for details on which file relates to which sample. 

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细菌是解析细胞分裂与全局调控程序运作机制的理想模型系统。在新月柄杆菌（Caulobacter crescentus）中，由一系列必需主调控因子组成的级联通路，精准调控DNA复制、细胞分裂以及不同细胞类型分化的时序性激活。其中，应答调控蛋白CtrA（response regulator CtrA）在协调上述所有功能中发挥核心作用。本研究首次报道了一种新型调控因子CcnA的功能：它是一类定位于复制起点、受CtrA调控并参与CtrA自身积累的细胞周期依赖性非编码RNA（ncRNA）。此外，CcnA还可能通过与S期调控因子GcrA的5'非翻译区（5'-UTR）结合，抑制其翻译过程。本研究通过体外实验与诱变实验，提出CcnA的作用机制：通过游离（针对ctrA）或隔离（针对gcrA）靶基因的核糖体结合位点（RBS）实现调控。最后，该调控通路的功能可能在其他生物中保守存在。

# 数据来源：《新月柄杆菌中非编码RNA CcnA调控主细胞周期因子CtrA与GcrA》
[https://doi.org/10.5061/dryad.4mw6m909k]

## 数据与文件结构说明
本数据集包含Fastq格式文件，相关样本与文件的对应关系请查阅`metadata.txt`文件。

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