Taxonomic and functional diversity of aquatic heterotrophs is sustained by dissolved organic matter chemodiversity

With this study, we advance the understanding of how specific aquatic heterotrophic bacteria act as central agents in the microbial loop. We use cultivated model communities developed from lake bacterioplankton under selection (i) in coculture with the cyanobacterium Microcystis aeruginosa (ii) growing on DOM produced from an axenic culture of M. aeruginosa, and (iii) growing in ambient lake DOM. After dilutions and serial transfer, cultivated model communities were established and characterized with a powerful combination of genome-resolved metagenomics and high-resolution untargeted metabolite analysis. This repository contains all metagenome-assembled genomes (MAGs) used in the study. The study is currently in bioRxiv https://www.biorxiv.org/content/10.1101/2022.03.21.485019v1

Each entry in the database corresponds to a MAG assembled from a cultured model community from Lake Erken. We had 33 cultures, 5 originated from inoculation on spent media from axenic M. aeruginosa (DOM$$_bin-**.fasta, where $$ is the numnber of the culture and ** is the MAG number), 21 cultures originated from filtered sterilized lake water (ERK$$_bin-**.fasta, where $$ is the numnber of the culture and ** is the MAG number) and 7 cultures were established as co-cultures with M. aeruginosa (CO$$_bin-**.fasta, where $$ is the numnber of the culture and ** is the MAG number) or to a MAG assembled from Lake Erken (Erken$$_bin-**.fasta, where $$ is name of the sample and ** is the MAG number).

本研究深化了对特定水生异养细菌如何作为微生物环核心介导因子的认知。我们采用源自湖泊浮游细菌的可培养模式群落，分别在三种条件下开展筛选培养：(i) 与铜绿微囊藻（Microcystis aeruginosa）共培养；(ii) 利用铜绿微囊藻无菌培养物产生的溶解性有机质（DOM）进行培养；(iii) 在天然湖泊溶解性有机质中培养。经稀释与连续传代后，我们成功构建可培养模式群落，并结合基因组解析宏基因组学与高分辨率非靶向代谢组分析对其进行全面表征。本数据集仓库包含本研究中使用的全部宏基因组组装基因组（MAG）。本研究目前已发布于bioRxiv预印本平台：https://www.biorxiv.org/content/10.1101/2022.03.21.485019v1

本数据库中的每条条目，均对应一个从埃尔肯湖（Lake Erken）可培养模式群落中组装得到的宏基因组组装基因组（MAG）。本次研究共包含33株培养物：其中5株源自以铜绿微囊藻无菌培养废培养基为底物的接种培养（对应文件格式为DOM$$_bin-**.fasta，其中$$为培养物编号，**为MAG编号）；21株源自经过滤除菌的湖水培养基接种培养（对应文件格式为ERK$$_bin-**.fasta，其中$$为培养物编号，**为MAG编号）；另有7株培养物，要么为与铜绿微囊藻共培养的体系（对应文件格式为CO$$_bin-**.fasta，其中$$为培养物编号，**为MAG编号），要么为直接从埃尔肯湖样本中组装得到的宏基因组组装基因组（对应文件格式为Erken$$_bin-**.fasta，其中$$为样本名称，**为MAG编号）。