Characterisation of Cap-G binding and its role in post-mitotic neurons in Drosophila

In order to gain a greater understanding of the role of the condensin subunit Cap-G in post-mitotic neurons, we profiled the binding of Cap-G to chromatin in neurons (at different stages of maturation) and neural stem cells in Drosophila using Targeted DamID. We also performed RNA-seq on larval brains that had Cap-G was knocked-down specifically in neurons. Overall design: Profiling of Cap-G binding in neurons of Drosophila. Also RNA-seq profiling of Cap-G knockdown larval brains.

为深入解析浓缩蛋白（condensin）亚基Cap-G在有丝分裂后神经元中的功能，我们采用靶向DamID（Targeted DamID）技术，对果蝇体内不同成熟阶段的神经元及神经干细胞中Cap-G与染色质的结合谱进行了分析。此外，我们还对神经元中特异性敲低Cap-G的果蝇幼虫脑组织实施了RNA测序（RNA-seq）。
整体实验设计：对果蝇神经元内Cap-G的染色质结合情况进行谱分析；同时对Cap-G敲低的果蝇幼虫脑组织开展RNA-seq谱分析。